Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Alpha-2-Macroglobulin (A2M) Rabbit pAb (A1573)

Publications (3) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Alpha-2-Macroglobulin (A2M) Rabbit pAb (A1573)

Western blot analysis of various lysates, using Alpha-2-Macroglobulin(A2M) antibody (A1573) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name Alpha-2-Macroglobulin (A2M) Rabbit pAb
Catalog No. A1573
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1155-1474 of human Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M)) (NP_000005.2).
Sequence ALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA
Gene ID 2
Swiss prot P01023
Synonyms A2MD; CPAMD5; FWP007; S863-7; Alpha-2-Macroglobulin (A2M)
Calculated MW 163kDa
Observed MW 163kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse uterus, Mouse brain
Cellular location Secreted
Customer validation

IHC (Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Homo sapiens, Rattus norvegicus)

IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Alpha-2-Macroglobulin (A2M) Rabbit pAb images

ABclonal:Western blot - Alpha-2-Macroglobulin (A2M) Rabbit pAb (A1573)}

Western blot - Alpha-2-Macroglobulin (A2M) Rabbit pAb (A1573)

Western blot analysis of various lysates, using Alpha-2-Macroglobulin(A2M) antibody (A1573) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on A2M. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to A2M. (Distance between topics and target gene indicate popularity.) A2M

* Data provided by citexs.com, for reference only.

Publishing research using A1573? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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