Products / NGS Lib Prep Kits

ABclonal mRNA-seq Lib Prep kit

Summary
Catalog No: RK20302
Size: 24 RXN / 96 RXN

To buy,select size,quantity

Overview

ABclonal mRNA-Seq Lib Prep kit for illumina:
  • Compatible with illumina sequencing platform.
  • Compatible with total RNA from animals, plants, fungi organisms.
  • Initial total RNA amount: 10ng-1μg, recommended RNA RIN value ≥ 7.
  • This Kit contains RNA truncated adapter. RNA index primer is used to add sequencing index to each sample in the PCR step. Compared to the full-length adapter, truncated adapter has better ligation efficiency, avoiding the formation of adapter dimers in the PCR step.
  • ABclonal mRNA-seq Lib Prep kit for illumina contains mRNA Capture module, First Strand synthesis module, Second Strand synthesis module, DNA Lib Prep module and ABclonal RNA adapter module.
  • Each kit component must pass rigorous quality control standards, and for each new lot the entire set of reagents is functionally validated together by construction and sequencing of an indexed transcriptome library on the Illumina sequencing platform.

Components

Box Module Name Catalog No.
BOX1 Poly(A)mRNA purification module RK20341
BOX2 First Strand synthesis module (non-stranded) RK20353
Second Strand synthesis module RK20346
DNA Lib Prep module RK20347
BOX3 RNA Adapter module 24 index RK20345

Storage Conditions

Box1: 2-8℃Oligo d(T)25 Capture beads can NOT be stored at -20℃.

Box2: -20℃.

Box3: -20℃.

Required Materials not included

Agencourt AMPure XP beads
80% Ethanol (freshly prepared)
Magnetic Racks
Thermal Cycler

Experimental Workflow

Considerations
  • Please perform mRNA purification at room temperature, dilute RNA samples on ice, and proceed directly to the next experiment to avoid RNA degradation.
  • Please select the recommended RNA fragmentation conditions and size selection conditions for the adapter ligated DNA.
  • Please carefully add RNA index primer into PCR reaction to avoid cross contamination between DNA samples and reagents.

Ask Us About This Product

submit your question on RK20302 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

You Viewed