Product Type > Molecular Biology Reagents > Nucleic Acid Electrophoresis > DNA Ladder / Marker

ColorMixed Protein Marker 180 (10-180 kDa) (RM19001)

Publications (4) Datasheet

Catalog No.: RM19001

ABclonal:
ABclonal:

Background

The ColorMixed Protein Marker 180 is a ready to use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer).
This marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF nylon, or nitrocellulose) and for approximating the size of proteins.

Product Information

Storage Temperature 4°C for 3 months, -20°C for 2years

Notice

  • 1. Readyto use: Do NOT heat, dilute, or add reducing agents before Loading
  • 2. Reference bands: 75 kDa (red), 25 kDa (green)
  • 3. Loading Volume: 3-5 μL/well for mini gel; 1.5-2.5 μL/ well for Western transfer; 6-10 μL/ well for large gel
  • 4. Type of membranes: PVDF nylon, or nitrocellulose

Guide for Molecular Weight

Migration patterns and approximate MWs (kDa) of ColorMixed Protein Marker 180 in different electrophoresis conditions are listed below:

BandColorTRIS-GLYCINE / kDaBIS-TRIS (MOPS) / kDaBIS-TRIS (MES)/ kDa
1Blue180170170
2Blue140130130
3Blue1009393
4Red757072
5Blue605353
6Blue454142
7Blue353030
8Green252223
9Blue151414
10Blue10910

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* For research use only. Not for therapeutic or diagnostic purposes.

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