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Rat IL-1 beta ELISA Kit

Summary
Catalog No:RK00009
Size:96 T
Sample:100 ul
Instrument:Microplate Reader
Detection:Colorimetric
Assay Time:4.0 hours
Conjugate:HRP
Precautions:Sandwich ELISA
ABclonal:Rat IL-1 beta ELISA Kit

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Additional information

RK00009Rat IL-1 beta ELISA Kit (RK00009) suitable for serum, plasma, cell culture supernatants and urine in Rat. 100% guaranteed. ABclonal provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for the competitive price.
SAMPLE TYPE100 ul
CROSS REACTS WITHRat
PRODUCT OVERVIEWThe ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
INTENDED USEThis Rat IL-1 beta ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat IL-1 beta in the sample, this Rat IL-1 beta ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat IL-1 beta concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
STORAGE INSTRUCTIONSStore at 4°C
Standard Curve Range 62.50-4000 pg/mL
COMPONENTSMicrowell Plate coated with monoclonal antibody to rat IL1B
Biotin-Conjugate anti-rat IL1B antibody
Streptavidin-HRP
Rat IL1B Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Specification
SAFETY NOTES1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

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