Product Type > Proteins

Recombinant Human CTLA-4/CD152 Protein (RP01044)

Datasheet

Overview

Product name Recombinant Human CTLA-4/CD152 Protein
Catalog No. RP01044
Description Recombinant Human CTLA-4/CD152 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala37-Phe162) of human CTLA-4 (Accession #NP_005205.2) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized lpilimumab at 1 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 with a linear range of 6-18 ng/mL.
2.Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD152 (CTLA-4) Antibody at 1μg/mL (25 μL/well) can bind Human CTLA-4 with a linear range of 0.46-2.05 ng/mL.
Purity > 90% by SDS-PAGE.
Endotoxin < 1.0 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
Synonym CTLA4; ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot P16410
Gene ID 1493
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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