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Human IL25 Protein (Fc Tag)

Summary
Catalog No:10096-H01H
Swiss Port:
Gene ID:
Species:Human
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Human IL25 Protein (Fc Tag) Product information

SynonymIL17E
Protein ConstructionHigh quality, high purity and low endotoxin recombinant Human IL25 Protein (Fc Tag) (10096-H01H), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
SpeciesHuman
Expressed HostHuman Cells
ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Human IL25 Protein (Fc Tag) QC Testing

Purity> 95 % as determined by SDS-PAGE
Bio-Activity1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His (Cat:13091-H08H) at 10 μg/ml (100 μl/well) can bind human Fc-IL25 (Cat:10096-H01H), The EC50 of human Fc-IL25 (Cat:10096-H01H) is 1.7-4.2 μg/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL.
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
StabilitySamples are stable for up to twelve months from date of receipt at -70℃
Predicted N TerminalGlu 20
Molecule MassThe recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions.
Formulation

Human IL25 Protein (Fc Tag) Usage Guide

StorageStore it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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