|Synonym||IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2|
|Protein Construction||High quality, high purity and low endotoxin recombinant Human IL-1 beta / IL1B Protein (10139-HNAE), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.|
|Expressed Host||E. coli|
|Shipping||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Purity||> 95 % as determined by SDS-PAGE|
|Bio-Activity||1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. 3. Measured by its binding ability in a functional ELISA. 4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 μg/mL. 5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.|
|Endotoxin||Please contact us for more information.|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N Terminal||Met|
|Molecule Mass||The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.|
|Storage||Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
submit your question on 10139-HNAE below
During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M-F (excluding major holidays), please contact us.