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Human CCNE1 / Cyclin-E1 Protein (His & GST Tag)

Summary
Catalog No:10902-H20B
Swiss Port:
Gene ID:
Species:Human
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Human CCNE1 / Cyclin-E1 Protein (His & GST Tag) Product information

SynonymCCNE
Protein ConstructionHigh quality, high purity and low endotoxin recombinant Human CCNE1 / Cyclin-E1 Protein (His & GST Tag) (10902-H20B), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
SpeciesHuman
Expressed HostBaculovirus-Insect Cells
ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Human CCNE1 / Cyclin-E1 Protein (His & GST Tag) QC Testing

Purity> 82 % as determined by SDS-PAGE
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 (Cat:10902-H20B) at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 45.0-106.0 ng/ml.
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
StabilitySamples are stable for up to twelve months from date of receipt at -70℃
Predicted N TerminalMet
Molecule MassThe recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
Formulation

Human CCNE1 / Cyclin-E1 Protein (His & GST Tag) Usage Guide

StorageStore it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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