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Human CCNE1 / Cyclin-E1 Protein

Summary
Catalog No:10902-HNCB
Swiss Port:
Gene ID:
Species:Human
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Human CCNE1 / Cyclin-E1 Protein Product information

SynonymCCNE
Protein ConstructionHigh quality, high purity and low endotoxin recombinant Human CCNE1 / Cyclin-E1 Protein (10902-HNCB), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
SpeciesHuman
Expressed HostBaculovirus-Insect Cells
ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Human CCNE1 / Cyclin-E1 Protein QC Testing

Purity> 90 % as determined by SDS-PAGE
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized human CCNE1 (Cat:10902-HNCB) at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 0.55-1.29 μg/ml.
Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
StabilitySamples are stable for up to twelve months from date of receipt at -70℃
Predicted N TerminalGly
Molecule MassThe secreted recombinant human CCNE1 consists of 412 amino acids and predicts a molecular mass of 47.2 KDa. The apparent molecular mass of the protein is approximately 48 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation

Human CCNE1 / Cyclin-E1 Protein Usage Guide

StorageStore it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ReconstitutionA hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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