Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZWINT Rabbit pAb (A10914)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - ZWINT Rabbit pAb (A10914)

Western blot analysis of various lysates using ZWINT Rabbit pAb (A10914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name ZWINT Rabbit pAb
Catalog No. A10914
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide of human ZWINT
Sequence Email for sequence
Gene ID 11130
Swiss prot O95229
Synonyms SIP30; ZWINT1; KNTC2AP; HZwint-1; ZWINT
Calculated MW 31kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Flow Cytometry    
Positive samples HT-29, Raji, 293T, Mouse kidney
Cellular location Chromosome, Nucleus, centromere, kinetochore
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ZWINT Rabbit pAb images

ABclonal:Western blot - ZWINT Rabbit pAb (A10914)}

Western blot - ZWINT Rabbit pAb (A10914)

Western blot analysis of various lysates using ZWINT Rabbit pAb (A10914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZWINT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZWINT. (Distance between topics and target gene indicate popularity.) ZWINT

* Data provided by citexs.com, for reference only.

Publishing research using A10914? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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