Summary
Catalog No:A7330
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - XRCC6 antibody (A7330)
ABclonal:Immunofluorescence - XRCC6 antibody (A7330)
ABclonal:Immunoprecipitation - 3ug XRCC6 antibody (A7330)

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Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).
SequencePYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD
Gene ID2547
Swiss ProtP12956
SynonymsCTC75, CTCBF, G22P1, KU70, ML8, TLAA
Calculated MW65kDa/69kDa
Observed MW70kDa

Applications

ReactivityHuman, Mouse, Monkey
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus

XRCC6 Polyclonal Antibody images

ABclonal:Western blot - XRCC6 antibody (A7330)

Western blot - XRCC6 antibody (A7330)

Western blot analysis of extracts of various cell lines, using XRCC6 antibody (A7330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - XRCC6 antibody (A7330)

Immunofluorescence - XRCC6 antibody (A7330)

Immunofluorescence analysis of A549 cells using XRCC6 antibody (A7330). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - 3ug XRCC6 antibody (A7330)

Immunoprecipitation - 3ug XRCC6 antibody (A7330)

Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug XRCC6 antibody (A7330). Western blot was performed from the immunoprecipitate using XRCC6 antibody (A7330) at a dilition of 1:1000.

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