Summary
Catalog No:A5862
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - XRCC5 antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)
ABclonal:Immunofluorescence - XRCC5 antibody (A5862)
ABclonal:Immunofluorescence - XRCC5 antibody (A5862)

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Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 543-732 of human XRCC5 (NP_066964.1).
SequenceKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI
Gene ID7520
Swiss ProtP13010
SynonymsKARP-1, KARP1, KU80, Ku86, KUB2, NFIV
Calculated MW82kDa
Observed MW100kDa

Applications

ReactivityHuman
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus, nucleolus
Customer Validation

WB (Human)

XRCC5 Polyclonal Antibody images

ABclonal:Western blot - XRCC5 antibody (A5862)

Western blot - XRCC5 antibody (A5862)

Western blot analysis of extracts of various cell lines, using XRCC5 antibody (A5862) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human stomach using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human breast cancer using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human breast cancer using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human stomach using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - XRCC5 Antibody (A5862)

Immunohistochemistry - XRCC5 Antibody (A5862)

Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC5 Antibody (A5862) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - XRCC5 antibody (A5862)

Immunofluorescence - XRCC5 antibody (A5862)

Immunofluorescence analysis of A549 cells using XRCC5 antibody (A5862). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XRCC5 antibody (A5862)

Immunofluorescence - XRCC5 antibody (A5862)

Immunofluorescence analysis of HeLa cells using XRCC5 antibody (A5862) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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