Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

XRCC3 Rabbit pAb (A2134)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - XRCC3 Rabbit pAb (A2134)

Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (A2134) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name XRCC3 Rabbit pAb
Catalog No. A2134
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
Sequence ILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH
Gene ID 7517
Swiss prot O43542
Synonyms CMM6; XRCC3
Calculated MW 38kDa
Observed MW 37kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HL-60
Cellular location Cytoplasm, Mitochondrion, Nucleus, perinuclear region
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

XRCC3 Rabbit pAb images

ABclonal:Western blot - XRCC3 Rabbit pAb (A2134)}

Western blot - XRCC3 Rabbit pAb (A2134)

Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (A2134) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on XRCC3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to XRCC3. (Distance between topics and target gene indicate popularity.) XRCC3

* Data provided by citexs.com, for reference only.

Publishing research using A2134? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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