Summary
Catalog No:A8354
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - XPC antibody (A8354)
ABclonal:Immunohistochemistry  - XPC antibody (A8354)

To buy,select size,quantity

Background

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).
SequenceGEVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQTEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIGCVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPVTDGYIVCEEFKDVLLTAWENEQAVIERKEKEKKEKRALGNWKLLAKGLLIRERLKRRYGPKSEAAAPHTDAGGGLSSDEEEGTSSQAEAARILAASWPQNREDEEKQKLKGGPKKTKREKKAAASHLFPFEQL
Gene ID7508
Swiss ProtQ01831
Synonymsp125, RAD4, XP3, XPCC
Calculated MW15kDa/101kDa/105kDa
Observed MW130kDa

Applications

ReactivityHuman
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesLO2, HeLa, 293T, HT-1080, Jurkat
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Human)

XPC Polyclonal Antibody images

ABclonal:Western blot - XPC antibody (A8354)

Western blot - XPC antibody (A8354)

Western blot analysis of extracts of various cell lines, using XPC antibody (A8354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.
ABclonal:Immunohistochemistry  - XPC antibody (A8354)

Immunohistochemistry - XPC antibody (A8354)

Immunohistochemistry of paraffin-embedded human liver cancer using XPC antibody (A8354) at dilution of 1:100 (40x lens).

Submit an review for A8354

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A8354 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A8354? Please let us know so that we can cite the reference in this datasheet.

You Viewed