Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VCP Rabbit pAb (A2795)

Review (1) Publications (7) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - VCP Rabbit pAb (A2795)

Western blot analysis of various lysates using VCP Rabbit pAb (A2795) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded rat kidney using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded human esophageal using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded mouse stomach using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for VCP Rabbit pAb(A2795)

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Overview

Product name VCP Rabbit pAb
Catalog No. A2795
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 460-770 of human VCP (NP_009057.1).
Sequence NPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGS
Gene ID 7415
Swiss prot P55072
Synonyms p97; TERA; CDC48; FTDALS6; VCP
Calculated MW 89kDa
Observed MW 90kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, K-562, SH-SY5Y, Mouse brain, Mouse spleen, Mouse kidney, Rat liver, Rat brain
Cellular location Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation

WB (mouse brainHomo sapiens, Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

VCP Rabbit pAb images

ABclonal:Western blot - VCP Rabbit pAb (A2795)}

Western blot - VCP Rabbit pAb (A2795)

Western blot analysis of various lysates using VCP Rabbit pAb (A2795) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)}

Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded rat kidney using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)}

Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded human esophageal using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A2795)}

Immunohistochemistry - VCP Rabbit pAb (A2795)

Immunohistochemistry analysis of VCP in paraffin-embedded mouse stomach using VCP Rabbit pAb (A2795) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on VCP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VCP. (Distance between topics and target gene indicate popularity.) VCP

* Data provided by citexs.com, for reference only.

Publishing research using A2795? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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