Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

VCP Mouse mAb (A0125)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - VCP Mouse mAb (A0125)

Western blot analysis of extracts of various cell lines, using VCP antibody (A0125).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name VCP Mouse mAb
Catalog No. A0125
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.

Immunogen information

Immunogen A synthetic peptide of human VCP
Sequence Email for sequence
Gene ID 7415
Swiss prot P55072
Synonyms p97; TERA; CDC48; FTDALS6; VCP
Calculated MW 89kDa
Observed MW 95kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HeLa, SH-SY5Y, Human brain
Cellular location Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation

WB (Homo sapiens)

IP (Homo sapiens)

Research Area

VCP Mouse mAb images

ABclonal:Western blot - VCP Mouse mAb (A0125)}

Western blot - VCP Mouse mAb (A0125)

Western blot analysis of extracts of various cell lines, using VCP antibody (A0125).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on VCP. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to VCP. (Distance between topics and target gene indicate popularity.) VCP

* Data provided by citexs.com, for reference only.

Publishing research using A0125? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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