Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

USP25 Mouse mAb (A1477)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - USP25 Mouse mAb (A1477)

Western blot analysis of extracts of BxPC-3 cells, using USP25 antibody (A1477).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name USP25 Mouse mAb
Catalog No. A1477
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).

Immunogen information

Immunogen A synthetic peptide of human USP25
Sequence Email for sequence
Gene ID 29761
Swiss prot Q9UHP3
Synonyms USP21; USP25
Calculated MW 122kDa
Observed MW 122kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples BxPC-3
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens)

IP (Homo sapiens)

USP25 Mouse mAb images

ABclonal:Western blot - USP25 Mouse mAb (A1477)}

Western blot - USP25 Mouse mAb (A1477)

Western blot analysis of extracts of BxPC-3 cells, using USP25 antibody (A1477).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on USP25. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to USP25. (Distance between topics and target gene indicate popularity.) USP25

* Data provided by citexs.com, for reference only.

Publishing research using A1477? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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