Summary
Catalog No:A2193
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - UBE2I antibody (A2193)
ABclonal:Immunohistochemistry  - UBE2I antibody (A2193)
ABclonal:Immunohistochemistry  - UBE2I antibody (A2193)

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Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
SequenceMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
Gene ID7329
Swiss ProtP63279
SynonymsC358B7.1, P18, UBC9
Calculated MW18kDa
Observed MW18kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, LO2, A-549, U-251MG, MCF-7, Mouse spleen, Mouse kidney, Rat spleen
Cellular LocationCytoplasm, Nucleus

UBE2I Polyclonal Antibody images

ABclonal:Western blot - UBE2I antibody (A2193)

Western blot - UBE2I antibody (A2193)

Western blot analysis of extracts of various cell lines, using UBE2I antibody (A2193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry  - UBE2I antibody (A2193)

Immunohistochemistry - UBE2I antibody (A2193)

Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (A2193) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - UBE2I antibody (A2193)

Immunohistochemistry - UBE2I antibody (A2193)

Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (A2193) at dilution of 1:100 (40x lens).

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