Summary
Catalog No:A3207
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - UBC antibody (A3207)
ABclonal:Immunohistochemistry  - UBC antibody (A3207)
ABclonal:Immunohistochemistry  - UBC antibody (A3207)

To buy,select size,quantity

Background

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
SequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
Gene ID7316
Swiss ProtP0CG48
SynonymsHMG20
Calculated MW77kDa
Observed MWRefer to Figures

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationCytoplasm, Nucleus
Customer Validation

IF (Mouse bv2)

WB (Human, Mouse)

UBC Polyclonal Antibody images

ABclonal:Western blot - UBC antibody (A3207)

Western blot - UBC antibody (A3207)

Western blot analysis of extracts of various cell lines, using UBC antibody (A3207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry  - UBC antibody (A3207)

Immunohistochemistry - UBC antibody (A3207)

Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (A3207).
ABclonal:Immunohistochemistry  - UBC antibody (A3207)

Immunohistochemistry - UBC antibody (A3207)

Immunohistochemistry of paraffin-embedded human liver using UBC antibody (A3207) at dilution of 1:100 (40x lens).

Submit an review for A3207

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A3207 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A3207? Please let us know so that we can cite the reference in this datasheet.

You Viewed