Products / Catalog Antibodies / Methylated Antibodies

TriMethyl-Histone H3-K79 pAb

Summary
Catalog No:A2369
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Dot-blot - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K79 antibody (A2369)
ABclonal:Chromatin immunoprecipitation - H3K79me3 antibody (A2369)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss ProtQ16695
SynonymsH3.4, H3/g, H3FT, H3t
Calculated MW15kDa
Observed MW18kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP, ChIP, ChIPseq
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus
Customer Validation

WB (HEK293T, HCT116, HeLa)

IF (Mouse testis)

TriMethyl-Histone H3-K79 pAb images

ABclonal:Western blot - TriMethyl-Histone H3-K79 antibody (A2369)

Western blot - TriMethyl-Histone H3-K79 antibody (A2369)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K79 antibody (A2369).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot-blot - TriMethyl-Histone H3-K79 antibody (A2369)

Dot-blot - TriMethyl-Histone H3-K79 antibody (A2369)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (A2369).
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K79 antibody (A2369) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry of paraffin-embedded rat kidney using TriMethyl-Histone H3-K79 antibody (A2369) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K79 antibody (A2369) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry - TriMethyl-Histone H3-K79 antibody (A2369)

Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K79 antibody (A2369) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K79 antibody (A2369)

Immunofluorescence - TriMethyl-Histone H3-K79 antibody (A2369)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K79 antibody (A2369). Blue: DAPI for nuclear staining.
ABclonal:Chromatin immunoprecipitation - H3K79me3 antibody (A2369)

Chromatin immunoprecipitation - H3K79me3 antibody (A2369)

Chromatin immunoprecipitation analysis extracts of 293 cell line, using H3K79me3 antibody (A2369) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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