Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

γ-Tubulin (A10611)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - γ-Tubulin (A10611)

Western blot analysis of extracts of various cell lines, using TUBG1 antibody (A10611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

You may also interested in:

Overview

Product name γ-Tubulin
Catalog No. A10611
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.

Immunogen information

Immunogen A synthetic peptide of human TUBG1
Sequence Email for sequence
Gene ID 7283
Swiss prot P23258
Synonyms TUBG; GCP-1; CDCBM4; TUBGCP1
Calculated MW 51kDa
Observed MW 51kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples A-431, MCF7, B cells, SKOV3, Mouse brain, Mouse lung, Mouse spleen, Rat heart
Cellular location Cytoplasm, centrosome, cytoskeleton, microtubule organizing center

Research Area

γ-Tubulin images

ABclonal:Western blot - γ-Tubulin (A10611)}

Western blot - γ-Tubulin (A10611)

Western blot analysis of extracts of various cell lines, using TUBG1 antibody (A10611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Inquire About This Product

Submit your question about A10611 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on TUBG1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBG1. (Distance between topics and target gene indicate popularity.) TUBG1

* Data provided by citexs.com, for reference only.

Publishing research using A10611? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Contact us to order