Summary
Catalog No:AC008
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - TUBB3 antibody (AC008)
ABclonal:Western blot - β-tubulin antibody (AC008)

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Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (NP_006077.2).
SequenceSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Gene ID10381
Swiss ProtQ13509
Synonymsbeta-4, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4
Calculated MW42kDa/50kDa
Observed MWRefer to Figures

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:5000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesBT474, HeLa, U251, MCF7, mouse brain, mouse spinal cord, rat brain, rat spinal cord
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

WB (Crab, Human, Oyster, Mouse)

IHC (Rat)

IF (Mouse)

TUBB3 Polyclonal Antibody images

ABclonal:Western blot - TUBB3 antibody (AC008)

Western blot - TUBB3 antibody (AC008)

Western blot analysis of extracts of various cell lines, using TUBB3 antibody (AC008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - β-tubulin antibody (AC008)

Western blot - β-tubulin antibody (AC008)

Western blot analysis of HeLa cell using β-tubulin antibody (AC008) at different dilution. Each lane was loaded with 10 ug cell lysate.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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