Summary
Catalog No:AC007
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - α-Tubulin antibody (AC007)
ABclonal:Western blot - α-Tubulin antibody (AC007)

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Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant protein of human Tubulin protein
SequenceEmail for sequence
Gene ID7277
Swiss ProtP68366
SynonymsALS22, H2-ALPHA, TUBA1
Calculated MW48kDa/49kDa
Observed MW55kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:2000 - 1:10000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHCT-116, 22Rv1, LOVO, 293T, HUVEC, U-87MG, HeLa, HepG2, A-549, Mouse lung, Mouse liver, Rat lung
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

WB (Pig, Mouse, Human, Mitten crab)

TUBA4A Polyclonal Antibody images

ABclonal:Western blot - α-Tubulin antibody (AC007)

Western blot - α-Tubulin antibody (AC007)

Western blot analysis of extracts of various cell lines, using α-Tubulin antibody (AC007).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - α-Tubulin antibody (AC007)

Western blot - α-Tubulin antibody (AC007)

Western blot analysis of extracts of various cell lines, using α-Tubulin antibody (AC007) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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