Summary
Catalog No:A0721
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - TSC1 antibody (A0721)

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Background

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenA synthetic peptide of human TSC1
SequenceEmail for sequence
Gene ID7248
Swiss ProtQ92574
SynonymsLAM, TSC
Calculated MW124kDa/129kDa
Observed MW140kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    
Positive samplesMouse brain
Cellular LocationCytoplasm, Membrane, Peripheral membrane protein
Customer Validation

IHC (Mouse)

TSC1 Monoclonal Antibody images

ABclonal:Western blot - TSC1 antibody (A0721)

Western blot - TSC1 antibody (A0721)

Western blot analysis of extracts of mouse brain, using TSC1 antibody (A0721).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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