Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

TSC1 Mouse mAb (A0721)

Publications (2) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - TSC1 Mouse mAb (A0721)

Western blot analysis of extracts of mouse brain, using TSC1 antibody (A0721).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name TSC1 Mouse mAb
Catalog No. A0721
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including TSC2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.

Immunogen information

Immunogen A synthetic peptide of human TSC1
Sequence Email for sequence
Gene ID 7248
Swiss prot Q92574
Synonyms LAM; TSC; TSC1
Calculated MW 130kDa
Observed MW 140kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples Mouse brain
Cellular location Cytoplasm, Membrane, Peripheral membrane protein
Customer validation

IHC (Mus musculus)

WB (Mus musculus)

Research Area

TSC1 Mouse mAb images

ABclonal:Western blot - TSC1 Mouse mAb (A0721)}

Western blot - TSC1 Mouse mAb (A0721)

Western blot analysis of extracts of mouse brain, using TSC1 antibody (A0721).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on TSC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TSC1. (Distance between topics and target gene indicate popularity.) TSC1

* Data provided by citexs.com, for reference only.

Publishing research using A0721? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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