Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

TRIM33 Mouse mAb (A9919)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - TRIM33 Mouse mAb (A9919)

Western blot analysis of various lysates using TRIM33 Mouse mAb (A9919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name TRIM33 Mouse mAb
Catalog No. A9919
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Immunogen information

Immunogen Recombinant protein of human TRIM33
Sequence Email for sequence
Gene ID 51592
Swiss prot Q9UPN9
Synonyms ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA; TRIM33
Calculated MW 123kDa
Observed MW 150kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MCF7, HeLa, HepG2
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

TRIM33 Mouse mAb images

ABclonal:Western blot - TRIM33 Mouse mAb (A9919)}

Western blot - TRIM33 Mouse mAb (A9919)

Western blot analysis of various lysates using TRIM33 Mouse mAb (A9919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TRIM33. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TRIM33. (Distance between topics and target gene indicate popularity.) TRIM33

* Data provided by citexs.com, for reference only.

Publishing research using A9919? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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