Summary
Catalog No:A0263
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - TP53 antibody (A0263)
ABclonal:Immunofluorescence - TP53 antibody (A0263)

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Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

ImmunogenRecombinant protein of human TP53
SequenceEmail for sequence
Gene ID7157
Swiss ProtP04637
SynonymsBCC7, LFS1, P53, TRP53
Calculated MW23-43kDa
Observed MW53kDa

Applications

ReactivityHuman
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer Validation

IF (SW480)

WB (Human cells, Human, Mouse)

TP53 Polyclonal Antibody images

ABclonal:Western blot - TP53 antibody (A0263)

Western blot - TP53 antibody (A0263)

Western blot analysis of extracts of various cell lines, using TP53 antibody (A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - TP53 antibody (A0263)

Immunofluorescence - TP53 antibody (A0263)

Immunofluorescence analysis of A549 cells using TP53 antibody (A0263).

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