Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TNF-α Rabbit pAb (A0277)

Review (1) Publications (58) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded rat spleen using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TNF-α Rabbit pAb(A0277)

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Overview

Product name TNF-α Rabbit pAb
Catalog No. A0277
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 77-233 of human TNF (NP_000585.2).
Sequence VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Gene ID 7124
Swiss prot P01375
Synonyms DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNF-α
Calculated MW 26kDa
Observed MW Refer to figures

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular location Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein
Customer validation

WB (Mouse embryonal fibroblast cellRattus norvegicus, Homo sapiens, Canis lupus familiaris, Rattus norvegicus, Mus musculus, Sus scrofa, Gallus gallus)

IHC (Mus musculus, Rattus norvegicus, Homo sapiens)

IF (Homo sapiens, Mus musculus, Rattus norvegicus)

ELISA (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TNF-α Rabbit pAb images

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry analysis of paraffin-embedded rat spleen using TNF-α Rabbit pAb (A0277) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277)}

Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TNF. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TNF. (Distance between topics and target gene indicate popularity.) TNF

* Data provided by citexs.com, for reference only.

Publishing research using A0277? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (3)

Proteins (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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