Summary
Catalog No:A0291
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - TGFB1 antibody (A0291)

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Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Immunogen information

ImmunogenRecombinant protein of human TGFB1
SequenceEmail for sequence
Gene ID7040
Swiss ProtP01137
SynonymsCED, DPD1, LAP, TGFB, TGFbeta
Calculated MW44kDa
Observed MW40kDa

Applications

ReactivityHuman
Tested applicationsWB, IHC, FC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
FC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesA-549, HepG2
Cellular LocationSecreted, extracellular matrix, extracellular space
Customer Validation

WB (Murine cells, KYSE410)

IHC (Human)

TGFB1 Monoclonal Antibody images

ABclonal:Western blot - TGFB1 antibody (A0291)

Western blot - TGFB1 antibody (A0291)

Western blot analysis of extracts of various cell lines, using TGFB1 antibody (A0291).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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