Summary
Catalog No:A2122
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - STK11 antibody (A2122)
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)
ABclonal:Immunofluorescence - STK11 antibody (A2122)

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Background

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 134-433 of human STK11 (NP_000446.1).
SequenceCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPK
Gene ID6794
Swiss ProtQ15831
SynonymshLKB1, LKB1, PJS
Calculated MW45kDa/48kDa
Observed MW54kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Membrane, Mitochondrion, Nucleus
Customer Validation

WB (HeLa, Human,Mouse,Rat)

STK11 Polyclonal Antibody images

ABclonal:Western blot - STK11 antibody (A2122)

Western blot - STK11 antibody (A2122)

Western blot analysis of extracts of various cell lines, using STK11 antibody (A2122) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)

Immunohistochemistry - STK11 antibody (A2122)

Immunohistochemistry of paraffin-embedded human esophageal cancer using STK11 antibody (A2122) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)

Immunohistochemistry - STK11 antibody (A2122)

Immunohistochemistry of paraffin-embedded human stomach using STK11 antibody (A2122) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)

Immunohistochemistry - STK11 antibody (A2122)

Immunohistochemistry of paraffin-embedded human lung cancer using STK11 antibody (A2122) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)

Immunohistochemistry - STK11 antibody (A2122)

Immunohistochemistry of paraffin-embedded human colon carcinoma using STK11 antibody (A2122) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - STK11 antibody (A2122)

Immunohistochemistry - STK11 antibody (A2122)

Immunohistochemistry of paraffin-embedded human uterine cancer using STK11 antibody (A2122) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - STK11 antibody (A2122)

Immunofluorescence - STK11 antibody (A2122)

Immunofluorescence analysis of MCF-7 cells using STK11 antibody (A2122). Blue: DAPI for nuclear staining.

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