Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

STAT3 Mouse mAb (A11867)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - STAT3 Mouse mAb (A11867)

Western blot analysis of various lysates using STAT3 Mouse mAb (A11867).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name STAT3 Mouse mAb
Catalog No. A11867
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Immunogen information

Immunogen Recombinant protein of human STAT3
Sequence Email for sequence
Gene ID 6774
Swiss prot P40763
Synonyms APRF; HIES; ADMIO; ADMIO1; STAT3
Calculated MW 88kDa
Observed MW 95kDa

Applications

Reactivity Human, Mouse, Rat, Monkey
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • IP 1:20 - 1:50
  • FC 1:20 - 1:50
  • ChIP 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    Flow Cytometry    
Positive samples HeLa, NIH/3T3, PC-12, COS
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens)

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

STAT3 Mouse mAb images

ABclonal:Western blot - STAT3 Mouse mAb (A11867)}

Western blot - STAT3 Mouse mAb (A11867)

Western blot analysis of various lysates using STAT3 Mouse mAb (A11867).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on STAT3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to STAT3. (Distance between topics and target gene indicate popularity.) STAT3

* Data provided by citexs.com, for reference only.

Publishing research using A11867? Please let us know so that we can cite the reference in this datasheet.

Antibodies (12)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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