Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SQSTM1/p62 Rabbit pAb (A7758)

Reviews (8) Publications (93) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SQSTM1/p62 Rabbit pAb (A7758)

Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (A7758) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human breast cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human lung cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded mouse lung using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded rat stomach using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HeLa cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)

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Overview

Product name SQSTM1/p62 Rabbit pAb
Catalog No. A7758
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_003891.1).
Sequence MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID 8878
Swiss prot Q13501
Synonyms p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3; SQSTM1/p62
Calculated MW 48kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples A549, HeLa, 293T, PC-12
Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation

IF (U-87MG cellsHomo sapiens, Mus musculus, Homo sapiens, Other)

WB (Human lung cancer cell, PANC-1, A549, Mouse cells, HepG2, Human Isikawa Mus musculus, Sus scrofa, Homo sapiens, Rattus norvegicus, Mus musculus, Danio rerio, Bombyx mori, Spodoptera frugiperda, Ctenopharyngodon idellus, Gallus gallus, Ctenopharyngodon idella, Apostichopus japonicus, Horabagrus nigricollaris)

IHC (Squamous cell carcinomaRattus norvegicus, Mus musculus, Rattus norvegicus)

IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SQSTM1/p62 Rabbit pAb images

ABclonal:Western blot - SQSTM1/p62 Rabbit pAb (A7758)}

Western blot - SQSTM1/p62 Rabbit pAb (A7758)

Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (A7758) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human breast cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human lung cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded mouse lung using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded rat stomach using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)}

Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HeLa cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)}

Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SQSTM1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SQSTM1. (Distance between topics and target gene indicate popularity.) SQSTM1

* Data provided by citexs.com, for reference only.

Publishing research using A7758? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

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