Summary
Catalog No:A0561
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - SOX2 antibody (A0561)
ABclonal:Immunohistochemistry  - SOX2 antibody (A0561)
ABclonal:Immunohistochemistry  - SOX2 antibody (A0561)
ABclonal:Immunofluorescence - SOX2 antibody (A0561)

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Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Immunogen information

ImmunogenRecombinant protein of human SOX2
SequenceEmail for sequence
Gene ID6657
Swiss ProtP48431
SynonymsANOP3, MCOPS3
Calculated MW34kDa
Observed MW39kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationNucleus
Customer Validation

WB (Human)

IF (Human)

SOX2 Polyclonal Antibody images

ABclonal:Western blot - SOX2 antibody (A0561)

Western blot - SOX2 antibody (A0561)

Western blot analysis of extracts of various cell lines, using SOX2 antibody (A0561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - SOX2 antibody (A0561)

Immunohistochemistry - SOX2 antibody (A0561)

Immunohistochemistry of paraffin-embedded rat brain using SOX2 antibody (A0561) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - SOX2 antibody (A0561)

Immunohistochemistry - SOX2 antibody (A0561)

Immunohistochemistry of paraffin-embedded mouse brain using SOX2 antibody (A0561) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - SOX2 antibody (A0561)

Immunofluorescence - SOX2 antibody (A0561)

Immunofluorescence analysis of A549 cells using SOX2 antibody (A0561).

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