Summary
Catalog No:A1340
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - SOD2 antibody (A1340)
ABclonal:Immunohistochemistry  - SOD2 antibody (A1340)
ABclonal:Immunohistochemistry  - SOD2 antibody (A1340)

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Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

Immunogen information

ImmunogenRecombinant protein of human SOD2
SequenceEmail for sequence
Gene ID6648
Swiss ProtP04179
SynonymsIPO-B, IPOB, Mn-SOD, MNSOD, MVCD6
Calculated MW18kDa/19kDa/20kDa/24kDa
Observed MW23kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, HepG2, DU145, U-251MG, Mouse kidney, Mouse liver, Mouse heart, Mouse skeletal muscle
Cellular LocationMitochondrion matrix
Customer Validation

WB (NPCs, Mouse, Rat, Pig, Human)

SOD2 Polyclonal Antibody images

ABclonal:Western blot - SOD2 antibody (A1340)

Western blot - SOD2 antibody (A1340)

Western blot analysis of extracts of various cell lines, using SOD2 antibody (A1340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - SOD2 antibody (A1340)

Immunohistochemistry - SOD2 antibody (A1340)

Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody (A1340) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - SOD2 antibody (A1340)

Immunohistochemistry - SOD2 antibody (A1340)

Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody (A1340) at dilution of 1:100 (40x lens).

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