Summary
Catalog No:A6311
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - SOAT1 antibody (A6311)

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Background

The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4).
SequenceMVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLD
Gene ID6646
Swiss ProtP35610
SynonymsACACT, ACAT, ACAT-1, ACAT1, SOAT, STAT
Calculated MW57kDa/58kDa/64kDa
Observed MW65kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T
Cellular LocationEndoplasmic reticulum membrane, Multi-pass membrane protein
Customer Validation

WB (Mouse)

SOAT1 Polyclonal Antibody images

ABclonal:Western blot - SOAT1 antibody (A6311)

Western blot - SOAT1 antibody (A6311)

Western blot analysis of extracts of 293T cells, using SOAT1 antibody (A6311).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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