Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Slug Rabbit pAb (A0572)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Slug Rabbit pAb (A0572)

Western blot analysis of extracts of various cell lines, using Slug antibody (A0572).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Slug Rabbit pAb
Catalog No. A0572
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Immunogen information

Immunogen A synthetic Peptide of human SLUG
Sequence Email for sequence
Gene ID 6591
Swiss prot O43623
Synonyms SLUG; WS2D; SLUGH; SLUGH1; SNAIL2; Slug
Calculated MW 30kDa
Observed MW 32kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples PC-12, Mouse heart
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Rattus norvegicus)

Research Area

Slug Rabbit pAb images

ABclonal:Western blot - Slug Rabbit pAb (A0572)}

Western blot - Slug Rabbit pAb (A0572)

Western blot analysis of extracts of various cell lines, using Slug antibody (A0572).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on SNAI2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SNAI2. (Distance between topics and target gene indicate popularity.) SNAI2

* Data provided by citexs.com, for reference only.

Publishing research using A0572? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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