Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BAF57/SMARCE1 Rabbit pAb (A5361)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - BAF57/SMARCE1 Rabbit pAb (A5361)

Western blot analysis of extracts of various cell lines, using BAF57/BAF57/SMARCE1 antibody (A5361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using BAF57/BAF57/SMARCE1 Antibody (A5361) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BAF57/BAF57/SMARCE1 Antibody (A5361) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunofluorescence analysis of U2OS cells using BAF57/BAF57/SMARCE1 antibody (A5361). Blue: DAPI for nuclear staining.

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Overview

Product name BAF57/SMARCE1 Rabbit pAb
Catalog No. A5361
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57/BAF57/SMARCE1 (NP_003070.3).
Sequence MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE
Gene ID 6605
Swiss prot Q969G3
Synonyms CSS5; BAF57; BAF57/SMARCE1
Calculated MW 47kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples U-251MG, Jurkat
Cellular location Nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

BAF57/SMARCE1 Rabbit pAb images

ABclonal:Western blot - BAF57/SMARCE1 Rabbit pAb (A5361)}

Western blot - BAF57/SMARCE1 Rabbit pAb (A5361)

Western blot analysis of extracts of various cell lines, using BAF57/BAF57/SMARCE1 antibody (A5361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)}

Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using BAF57/BAF57/SMARCE1 Antibody (A5361) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)}

Immunohistochemistry - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BAF57/BAF57/SMARCE1 Antibody (A5361) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - BAF57/SMARCE1 Rabbit pAb (A5361)}

Immunofluorescence - BAF57/SMARCE1 Rabbit pAb (A5361)

Immunofluorescence analysis of U2OS cells using BAF57/BAF57/SMARCE1 antibody (A5361). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMARCE1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMARCE1. (Distance between topics and target gene indicate popularity.) SMARCE1

* Data provided by citexs.com, for reference only.

Publishing research using A5361? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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