Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SHC1 Rabbit pAb (A7725)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of Jurkat cells, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of various cell lines, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of various cell lines, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SHC1 Rabbit pAb (A7725)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SHC1 Rabbit pAb (A7725) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SHC1 Rabbit pAb (A7725)

Immunofluorescence analysis of A549 cells using SHC1 Rabbit pAb (A7725) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SHC1 Rabbit pAb (A7725)

Immunofluorescence analysis of U-2 OS cells using SHC1 Rabbit pAb (A7725) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name SHC1 Rabbit pAb
Catalog No. A7725
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 300-400 of human SHC1 (NP_001189788.1).
Sequence TTPGQYVLTGLQSG
Gene ID 6464
Swiss prot P29353
Synonyms SHC; SHCA; SHC1
Calculated MW 63kDa
Observed MW 66kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, C6, Mouse lung, Mouse spleen, Rat liver
Cellular location Cytoplasm, Mitochondrion, Mitochondrion matrix

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SHC1 Rabbit pAb images

ABclonal:Western blot - SHC1 Rabbit pAb (A7725)}

Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of Jurkat cells, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - SHC1 Rabbit pAb (A7725)}

Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of various cell lines, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - SHC1 Rabbit pAb (A7725)}

Western blot - SHC1 Rabbit pAb (A7725)

Western blot analysis of extracts of various cell lines, using SHC1 antibody (A7725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - SHC1 Rabbit pAb (A7725)}

Immunohistochemistry - SHC1 Rabbit pAb (A7725)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SHC1 Rabbit pAb (A7725) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SHC1 Rabbit pAb (A7725)}

Immunofluorescence - SHC1 Rabbit pAb (A7725)

Immunofluorescence analysis of A549 cells using SHC1 Rabbit pAb (A7725) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SHC1 Rabbit pAb (A7725)}

Immunofluorescence - SHC1 Rabbit pAb (A7725)

Immunofluorescence analysis of U-2 OS cells using SHC1 Rabbit pAb (A7725) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A7725 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on SHC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SHC1. (Distance between topics and target gene indicate popularity.) SHC1

* Data provided by citexs.com, for reference only.

Publishing research using A7725? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order