Product Type > Antibodies > Secondary Antibodies

Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

Flow cytometry: 1X10^6 RK13 cells (negative control, left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100, orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). Rhodamine Goat Anti-Rat IgG (H+L)(AS022, 1:200) was used as a secondary antibody.

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Overview

Product name Rhodamine-conjugated Goat anti-Rat IgG (H+L)
Catalog No. AS022
Host species Goat
Purification method Affinity purification
Isotype Rhodamine conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rat IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application key Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF (Mus musculus)

Rhodamine-conjugated Goat anti-Rat IgG (H+L) images

ABclonal:Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)}

Flow CytoMetry - Rhodamine-conjugated Goat anti-Rat IgG (H+L) (AS022)

Flow cytometry: 1X10^6 RK13 cells (negative control, left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100, orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). Rhodamine Goat Anti-Rat IgG (H+L)(AS022, 1:200) was used as a secondary antibody.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rat. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rat. (Distance between topics and target gene indicate popularity.) Goat Anti-Rat

* Data provided by citexs.com, for reference only.

Publishing research using AS022? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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