Product Type > Antibodies > Tag and Loading Control Antibodies

Rabbit anti Myc-Tag pAb (AE009)

Publications (14) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - Rabbit anti Myc-Tag pAb (AE009)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)

Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (AE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Rabbit anti Myc-Tag pAb antibody (AE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb antibody (AE009) at a dilution of 1:50000.

ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T-NLK-Myc cells using 3 μg Myc-Tag antibody (AE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (AE009) at a dilution of 1:20000.

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Overview

Product name Rabbit anti Myc-Tag pAb
Catalog No. AE009
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Immunogen information

Immunogen A synthetic peptide corresponding to Myc tag.
Sequence EQKLISEEDL
Gene ID
Swiss prot
Synonyms Myc; Myc tag; Myc-tag
Calculated MW
Observed MW 60kDa

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples 293T-NLK-Myc
Cellular location
Customer validation

WB (Homo sapiens, Gallus gallus, Arabidopsis thaliana, Solanum tuberosum, Oryctolagus cuniculus, Mus musculus)

Co-IP (Homo sapiens)

IHC (Sus scrofa, Mus musculus)

IP (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Rabbit anti Myc-Tag pAb images

ABclonal:Western blot - Rabbit anti Myc-Tag pAb (AE009)}

Western blot - Rabbit anti Myc-Tag pAb (AE009)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)}

Immunofluorescence - Rabbit anti Myc-Tag pAb (AE009)

Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (AE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)}

Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Rabbit anti Myc-Tag pAb antibody (AE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb antibody (AE009) at a dilution of 1:50000.
ABclonal:Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)}

Immunoprecipitation - Rabbit anti Myc-Tag pAb (AE009)

Immunoprecipitation analysis of 300 μg extracts of 293T-NLK-Myc cells using 3 μg Myc-Tag antibody (AE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (AE009) at a dilution of 1:20000.

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Related Targets

Based on existing publications, the following genes are closely related to research on Myc. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Myc. (Distance between topics and target gene indicate popularity.) Myc

* Data provided by citexs.com, for reference only.

Publishing research using AE009? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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