Summary
Catalog No:AE006
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - GST-tag (AE006)
ABclonal:Western blot - GST-tag antibody (AE006)

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Background

Immunogen information

ImmunogenRecombinant protein of human GST-Tag
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to Figures

Applications

Reactivity
Tested applicationsWB, IF, IP
Recommended DilutionWB 1:1000 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesGST fusion protein
Cellular Location
Customer Validation

WB (Snakehead, Mouse, Human)

Rabbit anti GST-Tag pAb images

ABclonal:Western blot - GST-tag (AE006)

Western blot - GST-tag (AE006)

Western blot analysis of GST-tagged protein using GST-tag (AE006) antibody at different dilution. Each lane was loaded with 5 ug protein.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - GST-tag antibody (AE006)

Western blot - GST-tag antibody (AE006)

Western blot analysis of over-expressed GST fusion protein using GST-tag antibody (AE006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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