Product Type > Antibodies > Tag and Loading Control Antibodies

Rabbit Control IgG (AC005)

Publications (165) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Immunoprecipitation - Rabbit Control IgG (AC005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063). Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061).A mock served as negative control using rabbit Control IgG(AC005)and over-expressed 293T cell lysate served as positive control.

ABclonal:Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa, using 5 μg of Rabbit Control IgG(AC005) and CTCF Rabbit pAb(A1133). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

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Overview

Product name Rabbit Control IgG
Catalog No. AC005
Host species Rabbit
Purification method Potein A/G purification
Isotype IgG

Background

The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer.

Immunogen information

Immunogen None
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cellsCh 5μg antibody for 10μg-15μg of Chromatin
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Immunoprecipitation    
Positive samples
Cellular location
Customer validation

IP (Homo sapiens, Sus scrofa, Mus musculus, Rattus norvegicus, Xanthonycticebus pygmaeus, Bos taurus, Oryctolagus cuniculus, Anatinae)

WB (Rattus norvegicus, Homo sapiens, Bos taurus, Mus musculus, Plutella xylostella, Arabidopsis thaliana, Glaesserella parasuis, Cyprinus carpio, Shrimp, Sus scrofa, Ctenopharyngodon idellus, Cyprinus carpio Linnaeus)

ChIP (Mus musculus, Rattus norvegicus, Homo sapiens, Oryza sativa L., Capra hircus)

IF (Homo sapiens, Mus musculus)

IHC (Mus musculus)

RIP (Mus musculus, Homo sapiens, Scapharca Broughtonii, Drosophila, Sus scrofa, Drosophila melanogaster)

Co-IP (Homo sapiens, Mus musculus, Penaeus vannamei, Rattus norvegicus, Cricetulus griseus)

chip (Drosophila melanogaster)

CHIP (Drosophila melanogaster, Mus musculus, Arabidopsis thaliana, Homo sapiens, Rattus norvegicus)

CO-IP (Homo sapiens, Mus musculus)

Chip (Homo sapiens)

ChIP-qPCR (Zea mays)

PCR (Mus musculus)

Hcp protein secretion assay (aeromonas hydrophila)

RIP Assay (Sus scrofa)

ChIP-seq (Mus musculus)

CUT&Tag (Mus musculus, Homo sapiens, Oryza sativa, Other)

iRIP-seq (Oryctolagus cuniculus)

WBChIP (Homo sapiensMus musculus)

Rabbit Control IgG images

ABclonal:Immunoprecipitation - Rabbit Control IgG (AC005)}

Immunoprecipitation - Rabbit Control IgG (AC005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063). Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061).A mock served as negative control using rabbit Control IgG(AC005)and over-expressed 293T cell lysate served as positive control.
ABclonal:Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)}

Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa, using 5 μg of Rabbit Control IgG(AC005) and CTCF Rabbit pAb(A1133). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

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Related Targets

Based on existing publications, the following genes are closely related to research on Control IgG. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to Control IgG. (Distance between topics and target gene indicate popularity.) Control IgG

* Data provided by citexs.com, for reference only.

Publishing research using AC005? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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