Summary
Catalog No:A2547
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - RELA antibody (A2547)
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)
ABclonal:Immunofluorescence - RELA antibody (A2547)

To buy,select size,quantity

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant protein of human RELA
SequenceEmail for sequence
Gene ID5970
Swiss ProtQ04206
SynonymsNFKB3, p65
Calculated MW58kDa/59kDa/60kDa
Observed MW70kDa

Applications

ReactivityHuman, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (RAW264.7, Mouse atherosclerosis arterial tissue, HCC827, U251, African green monkey, Human)

IF (Rat H9c2 cells)

ChIP (Human)

RELA Polyclonal Antibody images

ABclonal:Western blot - RELA antibody (A2547)

Western blot - RELA antibody (A2547)

Western blot analysis of extracts of various cell lines, using RELA antibody (A2547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)

Immunohistochemistry - RELA Antibody (A2547)

Immunohistochemistry of paraffin-embedded rat lung using RELA Antibody (A2547) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)

Immunohistochemistry - RELA Antibody (A2547)

Immunohistochemistry of paraffin-embedded rat kidney using RELA Antibody (A2547) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)

Immunohistochemistry - RELA Antibody (A2547)

Immunohistochemistry of paraffin-embedded human kidney cancer using RELA Antibody (A2547) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - RELA Antibody (A2547)

Immunohistochemistry - RELA Antibody (A2547)

Immunohistochemistry of paraffin-embedded human stomach using RELA Antibody (A2547) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - RELA antibody (A2547)

Immunofluorescence - RELA antibody (A2547)

Immunofluorescence analysis of U2OS cells using RELA antibody (A2547).

Submit an review for A2547

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A2547 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A2547? Please let us know so that we can cite the reference in this datasheet.

You Viewed