Summary
Catalog No:A6268
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - RAD51 antibody (A6268)

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Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAD51 (NP_002866.2).
SequenceEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDR
Gene ID5888
Swiss ProtQ06609
SynonymsBRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA
Calculated MW26kDa/31kDa/36kDa
Observed MW37kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-937, Mouse brain, Mouse ovary, Mouse testis, Rat testis
Cellular LocationCytoplasm, Mitochondrion matrix, Nucleus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region
Customer Validation

WB (Mouse adipose-derived stem cells, HCC827, Human)

IF (Mouse adipose-derived stem cells)

RAD51 Polyclonal Antibody images

ABclonal:Western blot - RAD51 antibody (A6268)

Western blot - RAD51 antibody (A6268)

Western blot analysis of extracts of various cell lines, using RAD51 antibody (A6268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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