Summary
Catalog No:A3078
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - RAD50 antibody (A3078)
ABclonal:Immunoprecipitation - 3ug RAD50 antibody (A3078)

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Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAD50 (NP_005723.2).
SequenceMSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSM
Gene ID10111
Swiss ProtQ92878
SynonymshRad50, NBSLD, RAD502
Calculated MW138kDa/153kDa/154kDa
Observed MW170kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IP
Recommended DilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesA-549, MCF-7, 293T, HepG2, NIH/3T3, Mouse testis, Mouse spleen, Mouse thymus, Rat testis
Cellular LocationChromosome, Nucleus, telomere
Customer Validation

WB (Mouse adipose-derived stem cells, HCC827, Human)

RAD50 Polyclonal Antibody images

ABclonal:Western blot - RAD50 antibody (A3078)

Western blot - RAD50 antibody (A3078)

Western blot analysis of extracts of various cell lines, using RAD50 antibody (A3078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunoprecipitation - 3ug RAD50 antibody (A3078)

Immunoprecipitation - 3ug RAD50 antibody (A3078)

Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug RAD50 antibody (A3078). Western blot was performed from the immunoprecipitate using RAD50 antibody (A3078) at a dilition of 1:500.

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