Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Publications (30) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (AP0115) at 1:1000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-mTOR-S2448 antibody (AP0115) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name Phospho-mTOR-S2448 Rabbit mAb
Catalog No. AP0115
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0094

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S2448 of human mTOR (P42345).
Sequence TDSYS
Gene ID 2475
Swiss prot P42345
Synonyms SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Phospho-mTOR-S2448
Calculated MW 289kDa
Observed MW 289kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB Human
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T+serum, 293T+IGF-1, NIH/3T3+EGF
Cellular location Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

IHC (Mus musculus)

WB (Spodoptera frugiperda, Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-mTOR-S2448 Rabbit mAb images

ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)}

Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (AP0115) at 1:1000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)}

Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-mTOR-S2448 antibody (AP0115) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MTOR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MTOR. (Distance between topics and target gene indicate popularity.) MTOR

* Data provided by citexs.com, for reference only.

Publishing research using AP0115? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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