Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Myc-S62 Rabbit mAb (AP0989)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-c-Myc-S62 Rabbit mAb (AP0989)

Western blot analysis of extracts of various cell lines, using Phospho-c-Myc-S62 Rabbit mAb (AP0989) at 1:1000 dilution.293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

You may also interested in:

Overview

Product name Phospho-c-Myc-S62 Rabbit mAb
Catalog No. AP0989
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC1533

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S62 of human c-Myc (NP_002458.2).
Sequence PLSPS
Gene ID 4609
Swiss prot P01106
Synonyms MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S62
Calculated MW 51kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse
Tested applications Testing results
WB HumanMouse
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, NIH/3T3cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Cellular location Nucleus, nucleolus, nucleoplasm
Customer validation

WB (Mus musculus, Homo sapiensMus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Myc-S62 Rabbit mAb images

ABclonal:Western blot - Phospho-c-Myc-S62 Rabbit mAb (AP0989)}

Western blot - Phospho-c-Myc-S62 Rabbit mAb (AP0989)

Western blot analysis of extracts of various cell lines, using Phospho-c-Myc-S62 Rabbit mAb (AP0989) at 1:1000 dilution.293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Inquire About This Product

Submit your question about AP0989 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MYC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYC. (Distance between topics and target gene indicate popularity.) MYC

* Data provided by citexs.com, for reference only.

Publishing research using AP0989? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order