Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MYL12A-S18 Rabbit pAb (AP0412)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-MYL12A-S18 Rabbit pAb (AP0412)

Western blot analysis of extracts from 293 cells untreated or treated with starvation using Phospho-MYL12A-S18 antibody (AP0412).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MYL12A-S18 Rabbit pAb
Catalog No. AP0412
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S18 of human MYL12A
Sequence Email for sequence
Gene ID 10627
Swiss prot P19105
Synonyms MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24; Phospho-MYL12A-S18
Calculated MW 20kDa
Observed MW 20kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293
Cellular location cytoplasm, cytosol, extracellular exosome, Z disc
Customer validation

WB (Mus musculus)

Research Area

Phospho-MYL12A-S18 Rabbit pAb images

ABclonal:Western blot - Phospho-MYL12A-S18 Rabbit pAb (AP0412)}

Western blot - Phospho-MYL12A-S18 Rabbit pAb (AP0412)

Western blot analysis of extracts from 293 cells untreated or treated with starvation using Phospho-MYL12A-S18 antibody (AP0412).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MYL12A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYL12A. (Distance between topics and target gene indicate popularity.) MYL12A

* Data provided by citexs.com, for reference only.

Publishing research using AP0412? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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