Summary
Catalog No:AP0063
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Phospho-MAP2K1-S298 antibody (AP0063)
ABclonal:Immunoprecipitation - 2.5ug Phospho-MAP2K1-S298 antibody (AP0063)

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Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Immunogen information

ImmunogenA phospho specific peptide corresponding to residues surrounding S298 of human MAP2K1
SequenceEmail for sequence
Gene ID5604
Swiss ProtQ02750
SynonymsCFC3, MAPKK1, MEK1, MKK1, PRKMK1
Calculated MW40kDa/43kDa
Observed MW43kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IP
Recommended DilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samples293, NIH/3T3
Cellular LocationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body
Customer Validation

WB (Human)

Phospho-MAP2K1-S298 pAb images

ABclonal:Western blot - Phospho-MAP2K1-S298 antibody (AP0063)

Western blot - Phospho-MAP2K1-S298 antibody (AP0063)

Western blot analysis of extracts of 293 and NIH/3T3 cells, using Phospho-MAP2K1-S298 antibody (AP0063) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunoprecipitation - 2.5ug Phospho-MAP2K1-S298 antibody (AP0063)

Immunoprecipitation - 2.5ug Phospho-MAP2K1-S298 antibody (AP0063)

Immunoprecipitation analysis of 200ug extracts of 293 cells treated by PMA using 2.5ug Phospho-MAP2K1-S298 antibody (AP0063). Western blot was performed from the immunoprecipitate using Phospho-MAP2K1-S298 antibody (AP0063) at a dilition of 1:1000.

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