Summary
Catalog No:AP0099
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Phospho-H2AFX-S139 antibody (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Immunogen information

ImmunogenA phospho specific peptide corresponding to residues surrounding S139 of human Histone H2A.x
SequenceEmail for sequence
Gene ID3014
Swiss ProtP16104
SynonymsH2A.X, H2A/X, H2AX
Calculated MW15kDa
Observed MW15kDa

Applications

ReactivityHuman
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat
Cellular LocationChromosome, Nucleus
Customer Validation

WB (N2A)

IF (Mouse bone marrow)

Phospho-H2AFX-S139 pAb images

ABclonal:Western blot - Phospho-H2AFX-S139 antibody (AP0099)

Western blot - Phospho-H2AFX-S139 antibody (AP0099)

Western blot analysis of extracts of Jurkat cells, using Phospho-H2AFX-S139 antibody (AP0099). Jurkat cells were treated by etoposide.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence analysis of U2OS cells using Phospho-H2AFX-S139 antibody (AP0099). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence analysis of U2OS cells using Phospho-H2AFX-S139 antibody (AP0099). Blue: DAPI for nuclear staining.

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