Products / Catalog Antibodies / Phosphorylated Antibodies

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

Summary
Catalog No:AP0472
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472)

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Background

Immunogen information

ImmunogenA phospho specific peptide corresponding to residues surrounding T202 of human ERK1
SequenceEmail for sequence
Gene ID5594/5595
Swiss ProtP28482/P27361
Synonyms
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW38kDa/41kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesJurkat
Cellular Location
Customer Validation

WB (MDA-MB-231, Raw264.7, Mouse splenocytes, C6, Pig, Murine, Human, Mouse, Rat)

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb images

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472)

Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472)

Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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